管政兵

职称：副教授，硕士生导师

学历/学位：博士

电话：0510-85327725

电子邮箱：guanzb@jiangnan.edu.cn

 1981年出生，2009.6~2013.10 校聘副教授，2013.11~至今 副教授，其中2018.8~2019.8任江苏省第十一批科技镇长团成员（挂职扬州仪征市）。主要从事发酵工程、酶工程、再造烟叶生物技术、微生物代谢工程等领域研究工作，以第一作者/通讯作者发表SCI论文30多篇（累计IF>110），授权国家发明专利11项，主持国家自然科学基金面上项目等科研项目14项，曾获江南大学优秀班主任、优秀本科毕业论文指导教师、江南大学教学成果奖等。

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【代表性论著】

[1] Zhang ZH, Liao YX, Deng XT, Guan ZB*. Semi-rational engineering of glucosamine-6-phosphate deaminase for catalytic synthesis of glucosamine from D-fructose. Enzyme and Microbial Technology, 2025, 183:110552.

[2] Guan ZB*, Deng XT, Zhang ZH, Xu GC, Cheng WL, Liao XR, Cai YJ. Engineering Glucosamine-6-Phosphate Synthase to Achieve Efficient One-Step Biosynthesis of Glucosamine. ACS Chem. Biol. 2024, 19:1237-1242.   

[3] Yang CX, Zhang D, Ma H, Guo XM, Yan N, Deng XT, Liao XR, Guan ZB*. Screening of Strain from Reconstituted Sheet Tobacco Making High‑Yield Enzyme and Aroma. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 2024, 94:641-651. 

[4] Yan N, Ma H, Yang CX, Liao XR, Guan ZB*. Improving the decolorization activity of Bacillus pumilus W3 CotA-laccase to Congo Red by rational modification. Enzyme and Microbial Technology, 2022, 155:109977.   

[5] Ma H, Xu KZ, Wang YJ, Yan N, Liao XR, Guan ZB*. Enhancing the decolorization activity of Bacillus pumilus W3 CotA-laccase to Reactive Black 5 by site-saturation mutagenesis. Applied Microbiology and Biotechnology, 2020, 104:9193-9204.  

[6] Wang YJ, Xu KZ, Ma H, Liao XR, Guo G, Tian F, Guan ZB*. Recombinant horseradish peroxidase C1A immobilized on hydrogel matrix for dye decolorization and its mechanism on acid blue 129 degradation. Applied Biochemistry and Biotechnology, 2020, 192:861-880.   

[7] Xu KZ, Wang HR, Wang YJ, Xia J, Ma H, Cai YJ, Liao XR, Guan ZB*. Enhancement in catalytic activity of CotA-laccase from Bacillus pumilus W3 via site-directed mutagenesis. Journal of Bioscience and Bioengineering, 2020, 129:405-411.                           

[8] Xu KZ, Ma H, Wang YJ, Cai YJ, Liao XR, Guan ZB*. Extracellular expression of mutant CotA-laccase SF in Escherichia coli and its degradation of malachite green. Ecotoxicology and Environmental Safety, 2020, 193:110335.   

[9] Xia J, Wang Q, Luo Q, Chen Y, Liao XR, Guan ZB*. Secretory expression and optimization of Bacillus pumilus CotA-laccase mutant GWLF in Pichia pastoris and its mechanism on evans blue degradation. Process Biochemistry, 2019, 78:33-41.

[10] Guan ZB*, Luo Q, Wang HR, Chen Y, Liao XR. Bacterial laccases: promising biological green tools for industrial applications. Cellular and Molecular Life Sciences, 2018, 75:3569-3592.

[11] Luo Q, Chen Y, Xia J, Wang KQ, Cai YJ, Liao XR, Guan ZB*. Functional expression enhancement of Bacillus pumilus W3 CotA-laccase mutant WLF through site-directed mutagenesis. Enzyme and Microbial Technology, 2018, 109:11-19. 

[12] Chen Y, Luo Q, Zhou W, Xie Z, Cai YJ, Liao XR, Guan ZB*. Improving the catalytic efficiency of Bacillus pumilus W3 CotA-laccase by site-directed mutagenesis. Applied Microbiology and Biotechnology, 2017, 101:1935-1944.   

[13] Zhou W, Guan ZB*, Chen Y, Zhang F, Cai YJ, Xu CW, Chen XS, Liao XR. Production of spore laccase from Bacillus pumilus W3 and its application in dye decolorization after immobilization. Water Science and Technology, 2017, 76:147-154.  

[14] Guan ZB*, Wang KQ, Shui Y, Liao XR. Establishment of a markerless multiple-gene deletion method based on Cre/loxP mutant system for Bacillus pumilus. Journal of Basic Microbiology, 2017, 57:1065-1068.

[15] Guan ZB, Yi HX, Zhao H, Shui Y, Cai YJ, Liao XR. Cell division cycle 2 participates in eyestalk ablation-induced ovarian maturation of Procambarus clarkii. Aquaculture, 2017, 468:115-119.

[16] Guan ZB, Yin J, Chen K, Shui Y, Cai YJ, Liao XR. The hepatopancreas and ovary are the sites of vitellogenin synthesis in female red swamp crayfish (Procambarus clarkii). Journal of Crustacean Biology, 2016, 36:637-641.

[17] Guan ZB, Cai YJ, Zhang YZ, Zhao H, Liao XR. Complete genome sequence of Bacillus pumilus W3: A strain exhibiting high laccase activity. Journal of Biotechnology, 2015, 207:8-9.

[18] Guan ZB, Shui Y, Song CM, Zhang N, Cai YJ, Liao XR. Efficient secretory production of CotA-laccase and its application in the decolorization and detoxification of industrial textile wastewater. Environmental Science and Pollution Research, 2015, 22:9515-9523.

[19] Guan ZB, Song CM, Zhang N, Zhou W, Xu CW, Zhou LX, Zhao H, Cai YJ, Liao XR. Overexpression, characterization, and dye-decolorizing ability of a thermostable, pH-stable, and organic solvent-tolerant laccase from Bacillus pumilus W3. Journal of Molecular Catalysis B, Enzymatic, 2014, 101:1-6.

[20] Guan ZB, Zhang N, Song CM, Zhou W, Zhou LX, Zhao H, Xu CW, Cai YJ, Liao XR. Molecular Cloning, Characterization, and Dye-Decolorizing Ability of a Temperature- and pH-Stable Laccase from Bacillus subtilis X1. Applied Biochemistry and Biotechnology, 2014, 172:1147-1157.

[21] Guan ZB, Shui Y, Liao XR, Xu ZH, Zhou X. Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the ovary of red swamp crayfish Procambarus clarkii. Aquaculture, 2014, 418-419:67-71.

[22] Guan ZB, Shui Y, Zhou X, Xu ZH, Zhao CY, Song CM, Liao XR. Participation of calmodulin (CaM) in ovarian maturation induced by eyestalk ablation in red swamp crayfish Procambarus clarkii. Aquaculture Research, 2013, 44:1625-1631.

[23] Guan ZB, Zhang ZZ, Cao Y, Chen LL, Xie GF, Lu J. Analysis and comparison of bacterial communities in two types of “wheat Qu”, the starter culture of Shaoxing rice wine, using nested PCR-DGGE. Journal of Institute Brewing, 2012, 118:127-132.

[24] Guan ZB, Shui Y, Lu J. Molecular identification of the gene encoding porcine tristetraprolin (TTP). Molecular and Cellular Biochemistry, 2010, 345:145-151.

[25] Guan ZB, Cai GL, Sun JY, Lu J. Identification and expression analysis of bovine ANGPTL gene family. Current Zoology, 2010, 56:444-452.

[26] Guan ZB, Shui Y, Zhang JX, Zhang SQ. Molecular cloning, genomic organization and expression analysis of the gene encoding bovine (Bos taurus) B-cell activating factor belonging to the TNF family (BAFF). Gene, 2008, 425:17-22.

[27] Guan ZB, Ye JL, Dan WB, Yao WJ, Zhang SQ. Cloning, expression and bioactivity of duck BAFF. Molecular Immunology, 2007, 44:1471-1476.

[28] Guan ZB, Dan WB, Shui Y, Ye JL, Zhang SQ. cDNA cloning, expression and bioactivity of porcine BAFF. Developmental and Comparative Immunology, 2007, 31:1211-1219.

[29] Guan ZB, Yao WJ, Ye JL, Dan WB, Shen JY, Zhang SQ. The construction and characterization of a bifunctional EGFP/sAPRIL fusion protein. Applied Microbiology and Biotechnology, 2007, 73:1114-1122.

[30] Guan ZB, Shui Y, Zhang SQ. Two related ligands of the TNF family, BAFF and APRIL, in rabbit: Molecular cloning, 3D modeling, and tissue distribution. Cytokine, 2007, 39:192-200.

[31] 张登, 杨春雪, 马慧, 廖祥儒, 管政兵*. 再造烟叶生产过程物料微生物群落结构分析. 烟草科技,  2022, 55:1-9.

[32] 许开忠, 王雅静, 马慧, 蔡宇杰, 廖祥儒, 管政兵*. 重组短小芽孢杆菌CotA漆酶的酶学性质及发酵优化研究. 食品与生物技术学报, 2020, 39:28-34.

[33] 周稳, 管政兵*, 蔡宇杰, 宋晨萌, 廖祥儒. 芽孢杆菌漆酶的研究进展. 微生物学通报, 2015, 42: 1372-1383.

【科研项目】

[1] 国家自然科学基金面上项目（主持）

[2] 国家自然科学青年基金项目（主持）

[3] 教育部博士点基金项目（主持）

[4] 生物催化与酶工程国家重点实验室开放课题（主持）

[5] 工业生物技术教育部重点实验室开放课题（主持）

[6] 中央高校基本科研业务费自主科研项目（主持）

[7] 水科院淡水渔业研究中心合作项目（主持）

[8] 企业合作项目6项（主持）

【代表性授权专利】

[1] 管政兵，许开忠，廖祥儒。一种催化效率提高的漆酶 CotA 及其应用。专利授权号：ZL201811234782.X

[2] 管政兵，夏静，廖祥儒。一种异源重组毕赤酵母工程菌 GS115-pPIC9K-LacGWLF及其应用。 专利授权号：ZL201811266756.5

[3] 管政兵，陈宇，廖祥儒，罗权，陈康。一种催化活性提高的短小芽孢杆菌CotA漆酶突变体及其制备方法。专利授权号：ZL201510716645.X

[4] 管政兵，廖祥儒，宋晨萌，张宁。一种高效表达枯草芽孢杆菌漆酶的基因工程菌及其应用。专利授权号：ZL201310280822.5

[5] 管政兵，周稳，廖祥儒，蔡宇杰，伊海希。一种磁性固定化芽孢漆酶及其制备方法与应用。专利授权号：ZL201510493134.6

[6] 管政兵，周稳，廖祥儒，蔡宇杰，袁冬。一种以废弃海绵为载体的固定化芽孢漆酶及其制备方法。专利授权号：ZL201510456440.2

[7] 管政兵，陈宇，廖祥儒，蔡宇杰，赵宏。一种底物特异性提高的短小芽孢杆菌CotA漆酶突变体及制备方法。专利授权号：ZL201510718557.3

[8] 管政兵，周稳，廖祥儒，蔡宇杰。一种固定化芽孢漆酶及其制备与应用。专利授权号：ZL201510166951.0

[9] 管政兵，陈宇，廖祥儒，蔡宇杰，廖元元。一种通过复合点突变改造的高底物特异性短小芽孢杆菌CotA漆酶。专利授权号：ZL201510717404.7