许国超

职称：副教授、硕士生导师

学历/学位：研究生/工学博士

电话：13661841332

电子邮箱：guochaoxu@jiangnan.edu.cn

主要研究工作包括：1) 高效生物催化元件的靶向挖掘和快速表征，2) 新功能生物催化剂的改造策略和分子设计，3) 系统生物催化合成手性中间体和天然产物，4) 计算化学解析酶反应的催化历程和选择性起源等。面向本科生讲授《生物催化与手性技术》和《分子生物学》等课程，指导多名学生参加大学生挑战杯和生命科学竞赛等项目。近五年，主要参与指导学生在J. Am. Chem. Soc., ACS Catal., Chem. Eng. J., Chem. Commun.等期刊发表SCI论文30余篇，其中15篇IF>5，累计影响因子>150，他引次数>500；主持和参与多项国家及江苏省自然科学基金、国家重点研发计划和企业横向课题。获得教育部高等教育科技进步二等奖和中国石油化学工业联合会等省部级及行业协会奖励2项。

2019-2020     加州大学洛杉矶分校，化学与生物化学系，访问学者（Kendall N Houk课题组）

2017-至今       江南大学，生物工程学院，副教授

2014-2017      江南大学，生物工程学院，校聘副教授

2008-2013      华东理工大学，生物化工专业，工学博士

2004-2008      山东农业大学，生物工程专业，工学学士

一、论文（论著）发表情况

1. 第一作者及通讯作者(近五年)

[1] Xu GC, Dai W, Wang Y, Zhang L, Sun ZW, Zhou JY, Ni Y, Molecular switch manipulating Prelog priority of an alcohol dehydrogenase toward bulky-bulky ketones, Molecular Catalysis, 2020, 484, 110741.

[2] Xu GC, Zhu C, Jia P, Li AT, Ni Y, Han RZ, Zhou JY, Ni Y, Engineering an Alcohol Dehydrogenase for Balancing kinetics in NADPH Regeneration with 1,4-Butanediol as a Greener Cosubstrate, ACS Sustainable Chemistry & Engineering, 2019, 7, 15706-15714.

[3] Xu GC, Wu AN, Xiao L, Han RZ, Ni Y, Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB, Biotechnology for Biofuels, 2019, 12, 164.

[4] Xu GC, Wang Y, Tang MH, Zhou JY, Zhao J, Han RZ, Ni Y, Hydroclassified combinatorial saturation mutagenesis: reshaping substrate binding pockets of KpADH for enantioselective reduction of bulky-bulky ketones, ACS Catalysis, 2018, 8, 8336-8345.

[5] Xu GC, Yu HL, Zhang XY, Xu JH, Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from Kluyveromyces thermotolerans, ACS Catalysis, 2012, 2, 2566–2571.

[6] Xu GC, Yu HL, Zhang ZJ, Xu JH, Stereocomplementary bioreduction of β-ketonitrile without ethylated byproduct, Organic Letters, 2013, 15, 5408–5411.

[7] Xu GC, Shang YP, Yu HL, Xu JH, Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins, Chemical Communications, 2015, 51, 15728–15731.

[8] Xu GC, Zhang YP, Wang Y, Ni Y, Genome hunting of carbonyl reductases from Candida glabrata for efficient preparation of chiral secondary alcohols, Bioresource Technology, 2018, 247, 553-560.

[9] Xu GC, Ding JC, Han RZ, Dong JJ, Ni Y, Enhancing cellulose accessibility of corn stover by deep eutectic solvent pretreatment for butanol fermentation, Bioresource Technology, 2016, 203, 364–369.

[10] Zhang Y, Pan J, Luan ZJ, Xu GC, Park S, Xu JH, Cloning and Characterization of a Novel Esterase from Rhodococcus sp. for Highly Enantioselective Synthesis of a Chiral Cilastatin Precursor, Applied and Environmental Microbiology, 2014, 80, 7348–7355.

[11] Wang Y, Dai W, Liu YM, Zhang ZW, Zhou JY, Xu GC, Ni Y, Fine tuning the enantioselectivity and substrate specificity of alcohol dehydrogenase from Kluyveromyces polysporus by single residue at 237, Catalysis Communications, 2018, 108, 1-6.

[12] Xu GC, Yu HL, Shang YP, Xu JH, Enantioselective bioreductive preparation of chiral halohydrins employing two newly identified stereocomplementary reductases, RSC Advances, 2015, 5, 22703–22711.

[13] Xu GC, Zhang LL, Ni Y, Enzymatic preparation of D-phenyllactic acid at high space-time yield with a novel phenylpyruvate reductase identified from Lactobacillus sp CGMCC 9967, Journal of Biotechnology, 2016, 222, 29–37. 

[14] Xu GC, Yu HL, Xu JH, Facile Access to Chiral Alcohols with Pharmaceutical Relevance Using a Ketoreductase Newly Mined from Pichia guilliermondii, Chinese Journal of Chemistry, 2013, 31, 349–354.

[15] Xu GC, Tang MH, Ni Y, Asymmetric synthesis of lipitor chiral intermediate using a robust carbonyl reductase at high substrate to catalyst ratio, Journal of Molecular Catalysis B:Enzymatic, 2016, 123, 67–72.

[16] Xu GC, Li L, Han RZ, Dong JJ, Ni Y, Characterization and soluble expression of D-hydantoinase from Pseudomonas fluorescens for the synthesis of D-amino acids, Applied Biochemistry and Biotechnology, 2016, 1, 1–15.

[17] Xu GC, Bian YQ, Han RZ, Dong JJ, Ni Y, Cloning, expression, and characterization of budC gene encoding meso-2,3-butanediol dehydrogenase from Bacillus licheniformis, Applied Biochemistry and Biotechnology, 2016, 178, 604–617.

2. 指导学生发表论文(近五年)

[1] Ni J, Xu GC, Dai W, Zhao YL, Ni Y, Hyperconjugation promoted by hydrogen bonding

between His98/His241 and a carboxyl group contributes to tyrosine decarboxylase catalysis, Catalysis Science & Technology, 2019, 9, 6222-6226.

[2] Gong L, Xu ZY, Dong JJ, Li H, Han RZ, Xu GC, Ni Y, Composite coal fly ash solid acid catalyst in synergy with chloride for biphasic preparation of furfural from corn stover hydrolysate, Bioresource Technology, 2019, 293, 122065.

[3] Jiang MY, Xu GC, Ni J, Zhang K, Dong JJ, Han RZ Ni Y, Improving Soluble Expression of Tyrosine Decarboxylase from Lactobacillus brevis for Tyramine Synthesis with High Total Turnover Number, Applied Biochemistry & Biotechnology, 2019, 188, 436-439.

[4] Zhou JY, Wang Y, Xu GC, Wu L, Han RZ, Schwaneberg U, Rao YJ, Zhao YL, Zhou JH, Ni Y, Structural insight into enantioselective inversion of an alcohol dehydrogenase reveals a ‘polar gate’ in stereorecognition of diaryl ketones, Journal of the American Chemical Society, 2018, 140, 12645-12654.

[5] Xing WR, Xu GC, Dong JJ, Han RZ, Ni Y, Novel dihydrogen-bonding deep eutectic solvents: Pretreatment of rice straw for butanol fermentation featuring enzyme recycling and high solvent yield, Chemical Engineering Journal, 2018, 333, 712-720.

[6] Zhang WX, Xu GC, Huang L, Pan J, Yu HL, Xu JH, Highly efficient synthesis of (R)-3-quinuclidinol in a space-time yield of 916 g·L–1·d–1 using a new bacterial reductase ArQR, Organic Letters, 2013, 15, 4917–4919.

[7] Liu YF, Xu GC, Han RZ, Dong JJ, Ni Y, Identification of D-carbamoylase for biocatalytic cascade synthesis of D-tryptophan featuring high enantioselectivity, Bioresource Technology, 2018, 249, 720-728.

[8] Ding JC, Xu GC, Han RZ, Ni Y, Biobutanol production from corn stover hydrolysate pretreated with recycled ionic liquid by Clostridium saccharobutylicum DSM 13864, Bioresource Technology, 2016, 199, 228–234.

[9] Zhou JY, Xu GC, Han RZ, Dong JJ, Zhang WG, Zhang RZ, Ni Y, Carbonyl group-dependent high-throughput screening and enzymatic characterization of diaromatic ketone reductase, Catalysis Science & Technology, 2016, 6, 6320–6327.

[10] Gong Y, Xu GC, Chen Q, Yin JG, Li CX, Xu JH, Iterative multitarget evolution dramatically enhances the enantioselectivity and catalytic efficiency of Bacillus subtilis esterase towards bulky benzoate esters of DL-menthol, Catalysis, Science & Technology, 2016, 6, 2370–2376.

[11] Zhu HX, Xu GC, Zhang K, Kong XD, Han RZ, Zhou JH, Ni Y, Crystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding, Scientific Reports, 2016, 6, 27779.

[12] Han RZ, Xu GC, Dong JJ, Ni Y, Arginine deiminase: recent advances in discovery, crystal structure, and protein engineering for improved properties as an anti-tumor drug, Applied Microbiology and Biotechnology, 2016, 100, 4747–4760.

[13] Cheng J, Xu GC, Han RZ, Dong JJ, Ni Y, Efficient access to L-phenylglycine using a newly identified amino acid dehydrogenase from Bacillus clausii, RSC Advances, 2016, 6, 80557–80563.

[14] Fan CW, Xu GC, Ma BD, Bai YP, Zhang J, Xu JH, A novel D-mandelate dehydrogenase used in three-enzyme cascade reaction for highly efficient synthesis of non-natural chiral amino acids, Journal of Biotechnology, 2015, 195, 67–71.

[15] Gong Y, Xu GC, Zheng GW, Li CX, Xu JH, Journal of Molecular Catalysis B:Enzymatic, 2014, 109, 1–8.

[16] Xue TY, Xu GC, Han RZ, Ni Y, Soluble expression of (+)-gamma-lactamase in Bacillus subtilis for the enantioselective preparation of Abacavir precursor, Applied Biochemistry and Biotechnology, 2015, 176, 1687–1699.

[17] Yin JG, Xu GC, Zheng GW, Xu JH, Cloning and characterization of an enantioselective l-menthyl benzoate hydrolase from Acinetobacter sp ECU2040, Applied Biochemistry and Biotechnology, 2015, 176, 1102–1113.

二、专利情况

共申请中国发明专利18件，授权发明专利3件，申请国际专利4件。

三、承担教学科研项目情况

1、国家重点研发计划“合成生物学”重点专项（2019YFA0906400），子课题负责人，2020-2024；

2、江苏省自然科学基金面上项目（BK20171135），主持，2017-2020；                  

3、国家自然科学基金面上项目（21776112），主要参与人，2018-2021；                                  

4、国家自然科学基金青年项目(21506073)，主持，已结题。

四、指导学生获奖情况

1、孙泽文，2019年第十二届中国酶工程学术研讨会（吉林长春），优秀墙报奖。

2、窦哲，IFIBiop2019（马来西亚，米里），优秀墙报奖。                        

3、吴彦霏，2019年上海生物工程研究生学术论坛（上海），优秀墙报奖。          

4、王岳，2018年发酵工程学术研讨会（江苏无锡），优秀墙报奖。                 

5、肖琳，2018年先进生物智造研究生论坛（天津），优秀墙报奖。                 

6、朱诚，2018年先进生物智造研究生论坛（天津），优秀墙报奖。                 

7、倪婕，2018年先进生物制造研究生论坛（天津），优秀墙报奖。

以上资料更新时间截止：2020年3月。