中西秀树

职称：professor, Doctoral supervisor

学历/学位：Postgraduate/ Doctor

电话：15852719485

电子邮箱：hidekinakanishi@aist.go.jphotmail.com

EDUCATION

Ph. D. 2001      Tokyo University of Agriculture and Technology, Tokyo, Japan.

M. Sc. 1998     Tokyo University of Agriculture and Technology, Tokyo, Japan

B. Sc. 1996      Tokyo University of Agriculture and Technology, Tokyo, Japan

PROFESSIONAL EXPERIENCE

2011 Jul. -                Professor

                                 Jiangnan University

                                 Wuxi, China

2009 Oct.                 Assistant Professor

        -2011 Mar.        Fukushima Medical University

                                 Fukushima, Japan

2007 Jul.                  Postdoctoral Research fellow

        -2009 Sep.        Fukushima Medical University (Dr. Ikuo Wada Lab)

                                 Fukushima, Japan

2006 Jul.                  Postdoctoral Research Scientist (Dr. Shinichiro Nishimura   

        -2007Jun.         Lab)

                                 Drug-Seeds Discovery Research Laboratory

                                 National Institute of Advanced Industrial Science    

                                 and Technology

                                 Sapporo, Japan

2001 Jul.                  Postdoctoral Associate (Aaron Neiman Lab)

       -2006 Jun.         Department of Biochemistry and Cell Biology

                                 State University of New York at Stony Brook

                                 Stony Brook, NY

2001 Apr.-Jun.         Postdoctoral Research Scientist (Yoshifumi Jigami Lab)

                                 Institute of Molecular and Cell Biology

                                 National Institute of Advanced Industrial Science

                                 and Technology

                                 Tsukuba, Japan

PUBLICATIONS

1. Li F, Yang G, Tachikawa H, Shao K, Yang Y, Gao X-D, Nakanishi H. Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT. (2020) J Gen Appl Microbiol. In press

2. Rao J, Zhang R, Liang H, Gao X-D, Nakanishi H, Xu Y. Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents. (2019) Microb Cell Fact. 18: 18. 

3. Zhao S-B, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao X-D, Fujita M. Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. (2019) Biochim Biophys Acta. 1863: 1147-1157.

4. Feng S, Zhang H, Xu S, Zhi C, Nakanishi H, Gao X-D. Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin. (2019) Mater Sci Eng C Mater Biol Appl. 96:552-560.

5. Liu X, Li Z, Chen Z, Wang N, Gao Y, Nakanishi H, Gao X-D. Production of L-Ribulose Using an Encapsulated L-Arabinose Isomerase in Yeast Spores. (2019) Journal of Agricultural and Food Chemistry. 67: 4868-4875.

6. Shao K, Wang Q, Wang N, Gao X-D, Nakanishi H. Construction of functional chimeras of syntaxin-1A and its yeast orthologue, and their application to the yeast cell-based assay for botulinum neurotoxin serotype C. (2019) Biochimica et Biophysica Acta. 1863: 0-UNSP129396. 

7. Xu X-X, Li S-T, Wang N, Kitajima T, Yoko-O T, Fujita M, Nakanishi H, Gao X-D. Structural and functional analysis of Alg1 b-1,4 mannosyltransferase reveals the physiological importance of its membrane topology. (2018) Glycobiology. 28: 741-753.

8. Feng S, Zhang H, Zhi C, Gao X-D, Nakanishi H. pH-responsive charge-reversal polymer-functionalized boron nitride nanospheres for intracellular doxorubicin delivery. (2018) Int J Nanomedicine. 13: 641-652.

9. Pan H-P, Wang N, Tachikawa H, Gao X-D, Nakanishi H. Osw2 is required for proper assembly of glucan and/or mannan layers of the yeast spore wall. (2018) J Biochem. 163: 293-304.

10. Bemena LD, Mukama O, Wang N, Gao X-D, Nakanishi H. Characterization of a yeast sporulation-specific P450 family protein, Dit2, using an in vitro assay to crosslink formyl tyrosine. (2018) J Biochem. 163: 123-131.

11. Li S-T, Wang N, Xu X-X, Fujita M, Nakanishi H, Kitajima T, Dean N, Gao X-D. Alternative routes for synthesis of N-linked glycans by the Alg2 mannosyltransferase. (2018) FASEB J. 32: 2492-2506.

12. Li A, Cai L, Chen Z, Wang M, Wang N, Nakanishi H, Gao X-D, Li Z. Recent advances in the synthesis of rare sugars using DHAP-dependent aldolases. (2017) Carbohydr Res. 452: 108-115. (Review)

13. Nakamura TS, Numajiri Y, Okumura Y, Hidaka J, Tanaka T, Inoue I, Suda Y, Takahashi T, Nakanishi H, Gao X-D, Neiman AM, Tachikawa H. Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. (2017) Mol Biol Cell. 28: 3881-895.

14. Pan H-P, Wang N, Tachikawa H, Nakanishi H, Gao X-D. b-1,6-glucan synthesis-associated genes are required for proper spore wall formation in Saccharomyces cerevisiae. (2017) Yeast. 34: 431-446.

15. Bemena LD, Mukama O, Neiman AM, Li Z, Gao X-D, Nakanishi H. In vitro reconstitution of the yeast spore wall dityrosine layer to disclose the mechanism of its assembly. (2017) J Biol Chem. 292: 15880-15891.

16. Nakanishi H, Li F, Han B, Arai S, Gao X-D. Yeast cells as an assay system for in vivo O-GlcNAc modification. (2017) Biochim Biophys Acta. 1861: 1159-1167.

17. Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Production of encapsulated creatinase using yeast spores. (2017) Bioengineered. 8: 411-419.

18. Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Consecutive hydrolysis of creatinine using creatininase and creatinase encapsulated in Saccharomyces cerevisiae spores. (2017) Biotechnology Letters. 39: 261-267.

19. Li S-T, Wang N, Xu S, Yin J, Nakanishi H, Dean N, Gao X-D. Quantitative study of yeast Alg1 b-1,4 mannosyltransferase activity, a key enzyme involved in protein N-glycosylation. (2017) Biochim Biophys Acta. 1861: 2934-2941.

20. Feng S, Zhang H, Yan T, Huang D, Zhi C, Nakanishi H, Gao X-D. Folate-conjugated boron nitride nanospheres for targeted delivery of anticancer drugs. (2016) Int J Nanomedicine. 11: 4573–4582. 

21. Xu S, Zhang G-Y, Zhang H, Kitajima T, Nakanishi H, Gao X-D. Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae. (2016) Microb Cell Fact. 15: 179.

22. Li Z, Qiao Y, Cai L, Nakanishi H, Gao X-D. Characterization of glycerol phosphate oxidase from Streptococcus pneumoniae and its application for ketose synthesis. (2015) Bioorg Med Chem Lett. 25: 504-507.

23. Li Z, Li Y, Duan S, Liu J, Yuan P, Nakanishi H, Gao X-D. Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores. (2015) J Ind Microbiol Biotechnol. 42: 1117-1128.

24. Okumura Y, Nakamura TS, Tanaka T, Inoue I, Suda Y, Takahashi T, Nakanishi H, Nakamura S, Gao X-D, Tachikawa H. The dysferlin domain-only protein, Spo73, is required for prospore membrane extension in Saccharomyces cerevisiae. (2015) mSphere. 1: E0038-15.

25. Mohamed LA, Tachikawa H, Gao X-D, Nakanishi H. Yeast cell-based analysis of human lactate dehydrogenase isoforms. (2015) J Biochem. 158: 467-476.

26. Li Z, Wu X, Cai L, Duan S, Liu J, Yuan P, Nakanishi H, Gao X-D. Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritime MSB8. (2015) Bioorg Med Chem Lett. 25: 3980-3983.

27. Zhang H, Tachikawa H, Gao X-D, Nakanishi H. Applied uses of yeast spores as chitosan beads. (2014) Appl Environ Microbiol. 80: 5098-5105.

28. Shi L, Li Z, Tachikawa H, Gao X-D, Nakanishi H. Microencapsulation of enzymes using yeast spores. (2014) Appl Environ Microbiol. 80: 4502-4510.

29. Sakurai S, Hashimoto H, Nakanishi H, Arai S, Wada Y, Sun-Wada GH, Wada I, Hatsuzawa K. SNAP-23 regulates phagosome formation and maturation in macrophages. (2012) Mol Biol Cell. 23: 4849-4863.

30. Elias EV, Quiroga R, Gottig N, Nakanishi H, Nash TE, Neiman AM, Lujan HD. Characterization of SNARES determines the absence of a typical Golgi apparaus in the ancient eukaryote Giardia lamblia. (2008) J Biol Chem. 283: 35996-36010.

31. Yang HJ, Nakanishi H, Liu S, McNew JA, Neiman AM. Binding interactions can control SNARE specificity in vivo. (2008) J Cell Biol. 183: 1089-1100.

32. Suda Y, Nakanishi H, Mathieson EM, Neiman AM. Alternative modes of organellar segregation during sporulation in Saccharomyces cerevisiae. (2007) Eukaryot Cell. 11: 2009-2017.

33.  Pablo-Hernando ME, Arnaiz-Pita Y, Nakanishi H, Dawson D, Del Rey F, Neiman AM, Vazquez de Aldana CR. Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae. (2007) Genetics. 177: 281-293.

34.  Zeniou-Meyer M, Zabari N, Ashery U, Chasserot-Golaz S, Haeberle AM, Demais V, Bailly Y, Gottfied I, Nakanishi H, Neiman AM, Du G, Frohman MA, Bader MF, Vitale N. Phospholipase D1 production of phosphatidic acid at the plasma membrane promotes exocytosis of large dense-core granule at a late stage. (2007) J Biol Chem. 282: 21746-21757.

35.  Nakanishi H, Suda Y, Neiman AM. Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae. (2007) J Cell Sci. 120: 908-916.

36.  Nakanishi H, Morishita M, Schwartz C, Coluccio A, Engebrecht JA, Neiman AM. Phospholipase D and the SNARE Sso1p are necessary for vesicle fusion during sporulation in yeast. (2006) J Cell Sci. 119: 1406-1415.

37.  Winters MJ, Lamson RE, Nakanishi H, Neiman AM, Pryciak PM. A membrane binding domain in the Ste5 scaffold synergizes with G_βγ binding to control localization and signaling in pheromone response. (2005) Mol Cell. 20: 21-32.

38.  Nakanishi H, de los Santos P, Neiman AM. Positive and negative regulation of a SNARE protein by control of intracellular localization. (2004) Mol Biol Cell. 15: 1802-1815.

39.  Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD⁺ synthetase that is functionally conserved in mammals. (2003) Yeast. 20: 995-1005.

40.  Nakanishi H, Nakayama K-I, Yokota A, Tachikawa H, Takahashi N, Jigami Y. Hut1 proteins identified in Saccharomyces cerevisiae and Schizosaccharomyces pombe are functional homologues involved in the protein folding process at the endoplasmic reticulum. (2001) Yeast. 18: 543-554.

41.  Tachikawa H, Funahashi W, Takeuchi Y, Nakanishi H, Nishihara R, Katoh S, Gao X-D, Mizunaga T, Fujimoto D. Overproduction of Mpd2p suppresses the lethality of protein disulfide isomerase depletion in a CXXC sequence dependent manner. (1997) Biochem Biophys Res Commun. 239: 710-714.

 HONORS  AND AWARDS

1998-2001 The Japan Scholarship Foundation Scholarship for University Students