石贵阳-正高--

国家级重点学科

一级学科博士学位授予点

“双一流”建设学科

师资队伍
Faculty and staff

张显

张显

职称:教授

学历/学位:研究生、博士

电话:13771401977

电子邮箱:zx@jiangnan.edu.cn

简介

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长期从事应用微生物选育、工业微生物代谢工程育种、工业/食品用酶开发等研究,主要承担微生物学(含实验)、微生物遗传育种(含实验)课程的讲授工作。近年来在生物法生产功能性碳水化合物、高值氨基酸、工业/食品用酶改良等方面开展多项工作。合作发表SCI论文50余篇,获授权国家发明专利60余项,美国专利3项。近年来,主持国家重点研发项目子课题、国家自然科学基金面上项目、省部级重点项目、企业委托合作项目等多项。获2018江苏省科学技术奖二等奖、2018中国商业联合会科技进步奖特等奖、2016中国轻工业联合会科学技术发明二等奖。

学习工作经历

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2022.06~至  今   江南大学,生物工程学院,教授

2017.06~至  今   江南大学,生物工程学院,硕士生导师

2016.09~2022.06   江南大学,生物工程学院,副教授

2014.03~2016.08   江南大学,生物工程学院,讲师

2007.09~2014.01   江南大学,发酵工程专业,工学博士

2003.09~2007.06   江南大学,生物科学专业,理学学士

其中:

2018.10~2019.10   美国俄亥俄州立大学,化学与生化分子工程系,访问学者

2020.07~2021.06   无锡市科学技术局,农村科技与社会发展处,副处长(挂职)



主要成果

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一、论文(论著)发表情况

近年来合作发表SCI论文60余篇(通讯作者;Δ共同第一作者)

1. Zhang XianΔ, Zhang RongzhenΔ, Bao Teng, Rao Zhiming, Yang Taowei, Xu Meijuan, Xu Zhenghong, Li Huazhong, Yang Shangtian. The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis [J]. Metabolic Engineering. 23: 34-41, 2014.

2. Zhang Xian, Rao Zhiming*, Li Jingjing, Zhou Junping, Yang Taowei, Xu Meijuan, Bao Teng, Zhao Xiaojing. Improving the acidic stability of Staphylococcus aureus α acetolactate decarboxylase in Bacillus subtilis by changing basic residues to acidic residues [J]. Amino Acids. 47(4):707–717, 2015.

3. Song Liu, Xian Zhang, Fei Liu, Meijuan Xu, Taowei Yang, Mengfei Long, Junping Zhou, Tolbert Osire, Shangtian Yang, Zhiming Rao. Designing of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Production of 1,2-Amino Alcohols from Epoxides. ACS Synthetic Biology. 2019, 8:734-743.  

4. Xian Zhang, Rumeng Han, Teng Bao, Xiaojing Zhao, Xiangfei Li, Manchi Zhu, Taowei Yang, Meijuan Xu, Minglong Shao, Youxi Zhao, Zhiming Rao. Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method. Microbial Cell Factories. 2019, 18:128. 

5. Xian Zhang, Manchi Zhu, Rumeng Han, Youxi Zhao, Kewei Chen, Kai Qian, Minglong Shao, Taowei Yang, Meijuan Xu, Jianzhong Xu, Zhiming Rao. A novel 3-phytosterone-9α-hydroxylase oxidation subunit and its application in bioconversion of 4-androstene-3,17-dione to 9α-hydroxy-4-androstene-3,17-dione coupling with a NADH regeneration formate dehydrogenase. Molecules. 2019, 24(14):2534.

6. Zhang XianΔ, Bao TengΔ, Rao Zhiming, Yang Taowei, Xu Zhenghong, Yang Shangtian, Li Huazhong. Two-stage pH Control Strategy Based on The pH Preference of Acetoin Reductase Regulates Acetoin and 2,3-butanediol Distribution in Bacillus subtilis [J]. PLoS One. 9(3): e91187, 2014. 

7. Zhang Xian, Zhang Rongzhen*, Bao Teng, Yang Taowei, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis [J]. J Ind Microbiol Biotechnol. 40(9):1067-1076, 2013. 

8. Zhang Xian, Yang Taowei, Lin Qing, Xu Meijuan, Xia Haifeng, Xu Zhenghong, Li Huazhong, Rao Zhiming*. Isolation and identification of an acetoin high production bacterium that can reverse transform 2,3-butanediol to acetoin at the decline phase of fermentation [J]. World J Microbiol Biotechnol. 27(12):2785-2790, 2011. 

9. Zhang Xian, Zhang Rongzhen*, Yang Taowei, Zhang Jing, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Mutation breeding of acetoin high producing Bacillus subtilis blocked in 2,3-butanediol dehydrogenase [J]. World J Microbiol Biotechnol. 29: 1783-1789, 2013. 

10. Xian Zhang, Zhiming* R, Zhang L, Meijuan X, Taowei Y. Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration. SpringerPlus. 5:1207, 2016.

11. Xian Zhang,  Dan Wu, Taowei Yang, Meijuan Xu, Zhiming Rao*. Over-expression of Mycobacterium neoaurum 3-ketosteroid-Δ1-dehydrogenase in Corynebacterium crenatum for efficient bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione. Electronic Journal of Biotechnology. 2016, 24: 84-90.

12. Zhang, Xian, Zhi Wang, Yimai Wang, Xu Li, Manchi Zhu, Hengwei Zhang, Meijuan Xu, Taowei Yang, and Zhiming Rao*. Heterologous Expression and Rational Design of l-asparaginase from Rhizomucor miehei to Improve Thermostability. Biology. 2021; 10(12):1346.

13. Zhang, Xian, Ruiqi Lu, Qiang Wang, Mengkai Hu, Zhiyue Li, Meijuan Xu, Taowei Yang, Rongzhen Zhang*, and Zhiming Rao*.  Production of d-Tagatose by Whole-Cell Conversion of Recombinant Bacillus subtilis in the Absence of Antibiotics. Biology. 2021; 10(12):1343.

14. Qiang WangΔ, Xian ZhangΔ, *, Kexin Ren, Rumeng Han, Ruiqi Lu, Teng Bao, Xuewei Pan, Taowei Yang, Meijuan Xu, and Zhiming Rao*.Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis. Biotechnology for Biofuels and Bioproducts.15, 87 (2022).

15. Teng BaoΔ, Xian ZhangΔ, Xiaojing Zhao, Zhiming Rao, Taowei Yang,Shangtian Yang. Regulation of the NADH pool and NADH/NADPH ratio redistributes acetoin and 2,3-butanediol proportion in Bacillus subtilis. Biotechnology Journal, 8(10): 1298-1306, 2015. 

16. Bao TengΔ, Zhang XianΔ, Rao Zhiming, Zhao Xiaojing, Zhang Rongzhen, Yang Taowei, Xu Zhenghong, Yang Shangtian. Efficient whole-cell biocatalyst for acetoin production with NAD+ regeneration system through homologous co-expression of 2,3-butanediol dehydrogenase and NADH oxidase in engineered Bacillus subtilis. PLoS One, 9(7): e102951, 2014. 

17. Xu Li, Xian Zhang, Shuqin Xu, Meijuan Xu, Taowei Yang, Li Wang, Huiling Zhang, Haitian Fang, Tolbert Osire, Zhiming Rao.Insight into the thermostability of thermophilic L-asparaginase and non-thermophilic L-asparaginase II through bioinformatics and structural analysis. Applied Microbiology and Biotechnology. 2019, 103(17):7055-7070 

18. Xian Zhang, Zhaoyang Xu, Song Liu, Kai Qian, Meijuan Xu, Taowei Yang, Jianzhong Xu, Zhiming Rao. Improving the Production of Salt-Tolerant Glutaminase by Integrating Multiple Copies of Mglu into the Protease and 16S rDNA Genes of Bacillus subtilis 168. Molecules. 2019, 24(3):592. 

19. Chunlin Tan, Xian Zhang, Zhijing Zhu, Meijuan Xu, Taowei Yang, Tolbert Osire, Shangtian Yang, Zhiming Rao. Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440. Microbial Cell Factories. 2019, 18(12):14. 

20. Xu Li, Shuqin Xu, Xian Zhang, Meijuan Xu, Taowei Yang, Li Wang, Huiling Zhang, Haitian Fang, Tolbert Osire, Shangtian Yang, Zhiming Rao. Design of a high-efficiency synthetic system for l-asparaginase production in Bacillus subtilis. Engineering in Life Sciences. 2019, 19(3):229-239. 

21. Samuel Niyomukiza, Bao Teng, Zhang Xian, Yang Taowei, Xu Meijuan, Li Xin, Komera Irene, Philibert Tuyishime, Rao Zhiming. Optimized whole cell biocatalyst fromacetoin to 2,3-butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineered Bacillus subtilis. J Chem Technol Biotechnol. 2017, 92(9):2477-2487.

22. Xu Li, Xian Zhang, Shuqin Xu, Hengwei Zhang, Meijuan Xu, Taowei Yang, Li Wang, Haifeng Qian, Huiling Zhang, Haitian Fang, Tolbert Osire, Zhiming Rao, Shangtian Yang. Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases. Scientific Reports. 2018, 8:7915.

23. Zhang, Hengwei, Mengkai Hu, Qing Wang, Fei Liu, Meijuan Xu, Xian Zhang, and Zhiming Rao. A Novel Salt-Tolerant L-Glutaminase: Efficient Functional Expression, Computer-Aided Design, and Application. Fermentation. 2022; 8(9):444.

24. Liu, Fei, Junping Zhou, Meijuan Xu, Taowei Yang, Minglong Shao, Xian Zhang, and Zhiming Rao.One-Pot Biocatalytic Preparation of Enantiopure Unusual α-Amino Acids from α-Hydroxy Acids via a Hydrogen-Borrowing Dual-Enzyme Cascade. Catalysts. 2020; 10(12):1470.

25. Fu, Weilai, Qiang Wang, Shuhui Chen, Yunshuang Wang, Yaru Wang, Peifeng Duan, Ganfeng Yi, Chao Liu, Xian Zhang, and Zhiming Rao. Isolation and Identification of an Efficient Aerobic Denitrifying Pseudomonas stutzeri Strain and Characterization of Its Nitrite Degradation.Catalysts.2021; 11(10):1214.

26. Minglong Shao, Xian Zhang, Zhiming Rao*, Meijuan Xu, Taowei Yang, Hui Li, Zhenghong Xu, Shangtian Yang. Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration.Green Chemistry. 2016, 18:1774-1784.

27. Rongzhen Zhang, Taowei Yang, Zhiming Rao*, Hongmei Sun, Meijuan Xu, Xian Zhang, Zhenghong Xu, Shangtian Yang. Efficient one-step Preparation of γ-Aminobutyric Acid from Glucose without Exogenous Cofactor by the Designed Corynebacterium glutamicum. Green Chemistry. 2014, 16: 4190-4197.

28. Man Zaiwei, Zhiming Rao*, Xu Meijuan, Guo Jing, Yang Taowei, Zhang Xian, Xu Zhenghong*. Improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Metabolic Engineering. 2016, 38:310.

29. Yang Taowei, Rao Zhiming*, Hu Guiyuan, Zhang Xian, Liu Mei, Dai Yue, Xu Meijuan, Xu Zhenghong, Yang Shangtian. Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels. Biotechnology for Biofuels. 8:129, 2015.

30. Taowei Yang, Zhiming Rao*, Xian Zhang, Meijuan Xu, Zhenghong Xu, Shang-Tian Yang. Metabolic engineering strategies for acetoin and 2,3-butanediol production: Advances and prospects, Critical Reviews in Biotechnology. 2017, 37(8): 990-1005.


二、专利情况

参与申报发明专利100余项(其中申报国际发明专利12项),获授权中国发明专利60余项。

获授权国际发明专利

1. Zhiming Rao, Junxian Zheng, Meijuan Xu, Taowei Yang, Xian Zhang. Signal peptide, L-glutamic acid synthesized using konjac flour and methods of using same. United States Patent, No.: US 10329592 B2, 2019.

2. Zhiming Rao, Zaiwei Man, Meijuan Xu, Taowei Yang, Xian Zhang. Method for increasing yield of L-arginine by knocking out flavin reductases. United States Patent, No.: US 10465218 B2, 2019.

获授权国家发明专利(5项代表作):

1. 张显, 饶志明, 李谞, 杨套伟, 徐美娟, 张恒维, 赵浩东, 刘鹏。一种L-天冬酰胺酶突变体及其构建方法。中国发明专利,授权号:ZL2017113289780

2. 张显, 饶志明, 杨套伟, 徐美娟, 韩如梦。一种稳定性提高的NADH氧化酶及其在乙偶姻生产中的应用。中国发明专利,授权号:ZL201711323996X

3. 饶志明,张显,杨套伟,徐美娟,李静静,张静,刘俊佳。利用调控蛋白ALsR适度加强枯草芽孢杆菌乙偶姻合成。中国发明专利,授权号: ZL20120360503.0.

4. 饶志明,杨套伟,张显,徐美娟,满在伟。利用一株安全菌株发酵生物柴油副产物粗甘油生产2,3-丁二醇。中国发明专利,授权号:ZL201210360637.2

5. 饶志明,杨套伟,张显,徐美娟,满在伟。通过加强辅酶循环再生速率提高Bacillus amyloliquefaciens 2,3-丁二醇产量。中国发明专利,授权号:ZL201310342415.2

参与申报的国际专利(5项代表作):

1. 饶志明,郑俊贤,徐美娟,杨套伟,张显。一种信号肽及其在利用魔芋粉合成L-谷氨酸及其高值化的应用。PCT/CN2015/095285

2. 饶志明,郑俊贤,徐美娟,杨套伟,张显。一种信号肽及其在利用魔芋粉合成L-精氨酸及其高值化的应用。PCT/CN2015/095286

3. 饶志明,王梅洲,徐美娟,张显,杨套伟。一种利用重组枯草芽孢杆菌全细胞转化生产L-鸟氨酸的方法。PCT/CN2015/094424

4. 饶志明,龙水清,张显,杨套伟,徐美娟。一种酶活提高的L-天冬酰胺酶突变体及其构建方法。PCT/CN2015/094554

5. 饶志明,邵明龙,张显,杨套伟,徐美娟。一种新金色分枝杆菌来源的甾酮C27-单加氧酶及其应用。PCT/CN2016/097110

三、承担教学科研项目情况

1. 国家自然科学基金-面上项目:转录因子SctR调控氧化葡萄糖酸杆菌耐有机溶剂的分子机制解析及耐溶剂底盘细胞构建(32171471),2022.01-2025.12

2. 国家重点研发项目子课题:迭代提升细胞性能创建高产氨基酸菌种库(2021YFC2100903/003),2021.07-2025.06

3. 国家重点研发项目子课题:数字细胞驱动的性能模拟及测试应用2020YFA0908304/002,2020.11-2025.10

4. 国家自然科学基金-青年基金:Bacillus subtilis芽孢合成期乙偶姻合成代谢流重排研究(31500065),2016.1-2018.12

5. “863”项目“有机酸生物制造”子课题:α-氨基丁酸合成多酶催化体系协同调控与修饰(2015AA021004-4),2015.1-2017.12

6. 宁夏自治区重点研发项目:乳糖组分的生物转化技术研究与益生产品开发(2020BFH02011),2020.01-2023.12

7. 山东省重大科技创新工程项目:生物法高效制备高值化功能性碳水化合物生物催化剂工业属性强化(2019JZZY020605-2),2019.01-2021.12

8. 江苏省自然科学基金-青年基金:枯草芽孢杆菌细胞衰亡期乙偶姻合成调控研究(BK20150142),2015.7-2018.6

9. 中国博士后第9批特别资助:枯草芽孢杆菌芽孢形成期乙偶姻合成调控及分子机制研究(2016T90421),2016.1-2017.12

10. 企业合作横向项目:高产γ-氨基丁酸微生物菌株的筛选及功能强化(210864)2021.06-2022.12

11. 中国博士后第57批面上一等资助(2015M570407):L-天冬酰胺酶热稳定性改造及分子机理研究,2015.1-2016.12

 

四、获奖情况(含指导学生获奖)

1. 2015年入选江苏省“双创博士”

2. 钝齿棒杆菌合成高值氨基酸系统代谢改造关键技术与应用,中国轻工业联合会科学技术发明奖,二等奖,(个人排名4/6),2016 (饶志明; 许正宏; 徐美娟; 张显; 杨套伟; 崔永涛)

3. 高效生产谷氨酸链氨基酸微生物细胞工厂构建关键技术及产业化,江苏省科技厅, 科学技术奖,省部二等奖,(个人排名5/9), 2018.11.2 (饶志明; 徐美娟; 许正宏; 杨套伟; 张显; 刘元涛; 宁健飞; 郑璞; 邵明龙)

4. 微生物高效生产谷氨酸链氨基酸关键技术及产业化, 中国商业联合会, 科技进步, 特等奖, (个人排名3/14),2018.12.19 (饶志明; 徐美娟; 张显; 刘元涛; 赵春光; 杨套伟; 邵明龙; 郑璞; 蔡立明; 孟刚; 李学朋; 程立坤; 陈晓双; 贾雪峰)

5. 江苏优秀毕业论文指导教师,2019,江苏省三等奖,(个人排名1/2)

6. 全国大学生生命科学竞赛一等奖指导教师,2018,一等奖,(个人排名1/1)

7. 全国大学生生命科学创新创业大赛指导教师,2020,三等奖,(个人排名1/1)


以上资料更新时间截止:2022年9月


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石贵阳-正高--