中西秀树
中西秀树
职称：professor, Doctoral supervisor
学历/学位：Postgraduate/ Doctor
电话：18921391657
电子邮箱：hideki@jiangnan.edu.cn
多年来以酵母细胞为研究对象，主要从事分子生物学，生物化学以及细胞遗传学等领域的研究。酵母作为一种良好的模式真核细胞，具有微生物的特征又保持着与人体细胞相似的基本生理过程。目前，团队致力于研究突触小泡融合机制在酵母中的重构，并开发构建良好的高通量筛选体系进而筛选可用于医疗和美容治疗的药物。我们还研究酵母和哺乳动物细胞葡萄糖代谢产物影响其从静息状态向生长状态转变的机制，这将为癌症和传染病的治疗提供有益的方法。此外，我们通过研究非专业吞噬细胞的吞噬作用，阐明其生理功能，为开发新的药物递送系统提供理论基础。
1992.3 日本（大阪）清风中学 毕业
1992.4-1996.3 日本东京农工大学 学士学位
1996.4-1998.3 日本东京农工大学 硕士学位
1998.4-2001.3 日本东京农工大学 博士学位
工作经历：
2001.4-2001.6 日本产业技术综合研究所 博士后研究员
2001.7-2006.6 美国纽约州立大学 研究员
2006.7-2007.6 日本国立产业合研究所（AIST）糖锁研究中心 助理研究员
2007.7-2011.5 日本福岛县立医科大学 助理教授
2011.5-至今 江南大学生物工程学院 教授
一、论文（论著）发表情况
部分代表性论文如下：
1、 Liu G, Yang Y, Yang G, Duan S, Yuan P, Zhang S, Li F, Gao XD, Nakanishi H. Triosephosphate isomerase and its product glyceraldehyde-3-phosphate are involved in the regulatory mechanism that suppresses exit from the quiescent state in yeast cells. (2022) Microbiol Spectr. 10(4):e0089722.
2、 Yang Y, Liu G, Li F, Carey LB, Sun C, Ling K, Tachikawa H, Fujita M, Gao XD, Nakanishi H. Receptor for advanced glycation end-products (RAGE) mediates phagocytosis in nonprofessional phagocytes. (2022) Commun Biol. 5(1):824.
3、 Liu G, Yan Y, Tachikawa T, Gao X-D, Nakanishi H. Application of yeast spores as β-glucan particles. (2022) Particuology. 71: 34-40
4、 Li Z, Li W, Wang Y, Chen Z, Nakanishi H, Xu X, Gao XD. Establishment of a Novel Cell Surface Display Platform Based on Natural "Chitosan Beads" of Yeast Spores. (2022) J Agric Food Chem. 70(24):7479-7489.
5、 Nakamura TS, Suda Y, Muneshige K, Fujieda Y, Okumura Y, Inoue I, Tanaka T, Takahashi T, Nakanishi H, Gao X-D, Okada Y, Neiman AM, Tachikawa H. Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension. (2021) PLoS Genet. 17(8):e1009727
6、 Liu G, Gao X-D, Nakanishi H. Unique properties of the S. cerevisiae spore wall and its applications. (2020) Trends in Glycoscience and Glycotechnology. 32:e189-193. (review)
7、 Shao K, Li F, Yang Y, Wang N, Gao X-D, Nakanishi H. Characteristics of SNARE proteins are defined by distinctive properties of SNARE motifs. (2020) Biochim Biophys Acta. 1864(10):129658.
8、 Li Z, Liu X, Nakanishi H, Gao X-D. Encapsulation of mannose-6-phosphate isomerase in yeast spores and its application in l-ribose production. (2020) J Agric Food Chem. 68(25):6892-6899.
9、 Basiony M, Yang Y, Liu G, Gao X-D, Nakanishi H.J. Studies on the Properties of the Sporulation Specific Protein Dit1 and its Product Formyl Tyrosine. (2020) J Fungi. 6: E77.
10、 Jin Y, Okamoto M, Chibana H, Liu G, Gao X-D, Nakanishi H. Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells. (2020) Yeast. 37(7-8):359-371.
11、 Li F, Yang G, Tachikawa H, Shao K, Yang Y, Gao X-D, Nakanishi H. Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT. (2020) J Gen Appl Microbiol. 67(1):33-41.
12、 Rao J, Zhang R, Liang H, Gao X-D, Nakanishi H, Xu Y. Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents. (2019) Microb Cell Fact. 18: 18.
13、 Zhao S-B, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao X-D, Fujita M. Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. (2019) Biochim Biophys Acta. 1863: 1147-1157.
14、 Feng S, Zhang H, Xu S, Zhi C, Nakanishi H, Gao X-D. Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin. (2019) Mater Sci Eng C Mater Biol Appl. 96:552-560.
15、 Liu X, Li Z, Chen Z, Wang N, Gao Y, Nakanishi H, Gao X-D. Production of L-Ribulose Using an Encapsulated L-Arabinose Isomerase in Yeast Spores. (2019) J Agric Food Chem. 67: 4868-4875.
16、 Shao K, Wang Q, Wang N, Gao X-D, Nakanishi H. Construction of functional chimeras of syntaxin-1A and its yeast orthologue, and their application to the yeast cell-based assay for botulinum neurotoxin serotype C. (2019) Biochimica et Biophysica Acta. 1863: 0-UNSP129396.
17、 Xu X-X, Li S-T, Wang N, Kitajima T, Yoko-O T, Fujita M, Nakanishi H, Gao X-D. Structural and functional analysis of Alg1 b-1,4 mannosyltransferase reveals the physiological importance of its membrane topology. (2018) Glycobiology. 28: 741-753.
18、 Feng S, Zhang H, Zhi C, Gao X-D, Nakanishi H. pH-responsive charge-reversal polymer-functionalized boron nitride nanospheres for intracellular doxorubicin delivery. (2018) Int J Nanomedicine. 13: 641-652.
19、 Pan H-P, Wang N, Tachikawa H, Gao X-D, Nakanishi H. Osw2 is required for proper assembly of glucan and/or mannan layers of the yeast spore wall. (2018) J Biochem. 163: 293-304.
20、 Bemena LD, Mukama O, Wang N, Gao X-D, Nakanishi H. Characterization of a yeast sporulation-specific P450 family protein, Dit2, using an in vitro assay to crosslink formyl tyrosine. (2018) J Biochem. 163: 123-131.
21、 Li S-T, Wang N, Xu X-X, Fujita M, Nakanishi H, Kitajima T, Dean N, Gao X-D. Alternative routes for synthesis of N-linked glycans by the Alg2 mannosyltransferase. (2018) FASEB J. 32: 2492-2506.
22、 Li A, Cai L, Chen Z, Wang M, Wang N, Nakanishi H, Gao X-D, Li Z. Recent advances in the synthesis of rare sugars using DHAP-dependent aldolases. (2017) Carbohydr Res. 452: 108-115. (Review)
23、 Nakamura TS, Numajiri Y, Okumura Y, Hidaka J, Tanaka T, Inoue I, Suda Y, Takahashi T, Nakanishi H, Gao X-D, Neiman AM, Tachikawa H. Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. (2017) Mol Biol Cell. 28: 3881-895.
24、 Pan H-P, Wang N, Tachikawa H, Nakanishi H, Gao X-D. b-1,6-glucan synthesis-associated genes are required for proper spore wall formation in Saccharomyces cerevisiae. (2017) Yeast. 34: 431-446.
25、 Bemena LD, Mukama O, Neiman AM, Li Z, Gao X-D, Nakanishi H. In vitro reconstitution of the yeast spore wall dityrosine layer to disclose the mechanism of its assembly. (2017) J Biol Chem. 292: 15880-15891.
26、 Nakanishi H, Li F, Han B, Arai S, Gao X-D. Yeast cells as an assay system for in vivo O-GlcNAc modification. (2017) Biochim Biophys Acta. 1861: 1159-1167.
27、 Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Production of encapsulated creatinase using yeast spores. (2017) Bioengineered. 8: 411-419.
28、 Kong J, Li Z, Zhang H, Gao X-D, Nakanishi H. Consecutive hydrolysis of creatinine using creatininase and creatinase encapsulated in Saccharomyces cerevisiae spores. (2017) Biotechnology Letters. 39: 261-267.
29、 Li S-T, Wang N, Xu S, Yin J, Nakanishi H, Dean N, Gao X-D. Quantitative study of yeast Alg1 b-1,4 mannosyltransferase activity, a key enzyme involved in protein N-glycosylation. (2017) Biochim Biophys Acta. 1861: 2934-2941.
30、 Feng S, Zhang H, Yan T, Huang D, Zhi C, Nakanishi H, Gao X-D. Folate-conjugated boron nitride nanospheres for targeted delivery of anticancer drugs. (2016) Int J Nanomedicine. 11: 4573–4582.
31、 Xu S, Zhang G-Y, Zhang H, Kitajima T, Nakanishi H, Gao X-D. Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae. (2016) Microb Cell Fact. 15: 179.
32、 Li Z, Qiao Y, Cai L, Nakanishi H, Gao X-D. Characterization of glycerol phosphate oxidase from Streptococcus pneumoniae and its application for ketose synthesis. (2015) Bioorg Med Chem Lett. 25: 504-507.
33、 Li Z, Li Y, Duan S, Liu J, Yuan P, Nakanishi H, Gao X-D. Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores. (2015) J Ind Microbiol Biotechnol. 42: 1117-1128.
34、 Okumura Y, Nakamura TS, Tanaka T, Inoue I, Suda Y, Takahashi T, Nakanishi H, Nakamura S, Gao X-D, Tachikawa H. The dysferlin domain-only protein, Spo73, is required for prospore membrane extension in Saccharomyces cerevisiae. (2015) mSphere. 1: E0038-15.
35、 Mohamed LA, Tachikawa H, Gao X-D, Nakanishi H. Yeast cell-based analysis of human lactate dehydrogenase isoforms. (2015) J Biochem. 158: 467-476.
36、 Li Z, Wu X, Cai L, Duan S, Liu J, Yuan P, Nakanishi H, Gao X-D. Enzymatic synthesis of rare sugars with l-rhamnulose-1-phosphate aldolase from Thermotoga maritime MSB8. (2015) Bioorg Med Chem Lett. 25: 3980-3983.
37、 Zhang H, Tachikawa H, Gao X-D, Nakanishi H. Applied uses of yeast spores as chitosan beads. (2014) Appl Environ Microbiol. 80: 5098-5105.
38、 Shi L, Li Z, Tachikawa H, Gao X-D, Nakanishi H. Microencapsulation of enzymes using yeast spores. (2014) Appl Environ Microbiol. 80: 4502-4510.
39、 Sakurai S, Hashimoto H, Nakanishi H, Arai S, Wada Y, Sun-Wada GH, Wada I, Hatsuzawa K. SNAP-23 regulates phagosome formation and maturation in macrophages. (2012) Mol Biol Cell. 23: 4849-4863.
40、 Elias EV, Quiroga R, Gottig N, Nakanishi H, Nash TE, Neiman AM, Lujan HD. Characterization of SNARES determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia. (2008) J Biol Chem. 283: 35996-36010.
41、 Yang HJ, Nakanishi H, Liu S, McNew JA, Neiman AM. Binding interactions can control SNARE specificity in vivo. (2008) J Cell Biol. 183: 1089-1100.
42、 Suda Y, Nakanishi H, Mathieson EM, Neiman AM. Alternative modes of organellar segregation during sporulation in Saccharomyces cerevisiae. (2007) Eukaryot Cell. 11: 2009-2017.
43、 Pablo-Hernando ME, Arnaiz-Pita Y, Nakanishi H, Dawson D, Del Rey F, Neiman AM, Vazquez de Aldana CR. Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae. (2007) Genetics. 177: 281-293.
44、 Zeniou-Meyer M, Zabari N, Ashery U, Chasserot-Golaz S, Haeberle AM, Demais V, Bailly Y, Gottfied I, Nakanishi H, Neiman AM, Du G, Frohman MA, Bader MF, Vitale N. Phospholipase D1 production of phosphatidic acid at the plasma membrane promotes exocytosis of large dense-core granule at a late stage. (2007) J Biol Chem. 282: 21746-21757.
45、 Nakanishi H, Suda Y, Neiman AM. Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae. (2007) J Cell Sci. 120: 908-916.
46、 Nakanishi H, Morishita M, Schwartz C, Coluccio A, Engebrecht JA, Neiman AM. Phospholipase D and the SNARE Sso1p are necessary for vesicle fusion during sporulation in yeast. (2006) J Cell Sci. 119: 1406-1415.
47、 Winters MJ, Lamson RE, Nakanishi H, Neiman AM, Pryciak PM. A membrane binding domain in the Ste5 scaffold synergizes with Gβγ binding to control localization and signaling in pheromone response. (2005) Mol Cell. 20: 21-32.
48、 Nakanishi H, de los Santos P, Neiman AM. Positive and negative regulation of a SNARE protein by control of intracellular localization. (2004) Mol Biol Cell. 15: 1802-1815.
49、 Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD+ synthetase that is functionally conserved in mammals. (2003) Yeast. 20: 995-1005.
50、 Nakanishi H, Nakayama K-I, Yokota A, Tachikawa H, Takahashi N, Jigami Y. Hut1 proteins identified in Saccharomyces cerevisiae and Schizosaccharomyces pombe are functional homologues involved in the protein folding process at the endoplasmic reticulum. (2001) Yeast. 18: 543-554.
51、 Tachikawa H, Funahashi W, Takeuchi Y, Nakanishi H, Nishihara R, Katoh S, Gao X-D, Mizunaga T, Fujimoto D. Overproduction of Mpd2p suppresses the lethality of protein disulfide isomerase depletion in a CXXC sequence dependent manner. (1997) Biochem Biophys Res Commun. 239: 710-714.
二、专利情况
1、chs3∆酿酒酵母孢子的应用及β-葡聚糖微球的制备方法，202111068850.1，
中西秀树，高晓冬，刘国玉，申请
2、Lactate dehydrogenase humanized Saccharomyces cerevisiae and its construction，PCT/CN2016/087449， 高晓冬，中西秀树，李子杰， 申请
3、一种以酿酒酵母孢子为载体的新型固定化酶的制备方法. ZL201310422568.8 中西秀树，高晓冬，张海妮，李子杰，授权
4、一种重组大肠杆菌全细胞转化合成D-阿洛酮糖的方法，201610821862.X，高晓冬，李子杰，李雪君，中西秀树，申请
5、一种乳酸脱氢酶人源化酿酒酵母及其构建方法，201510369294.X，高晓冬，中西秀树，李子杰，申请
6、一种利用酿酒酵母二酪氨酸层疏松型孢子固定化酶的方法，201410199369.X，高晓冬，中西秀树，施李兵，李子杰，申请
7，一种利用酿酒酵母孢子的微胶囊固定化酶的制备方法，201410199661.1，中西秀树，高晓冬，施李兵，李子杰，申请
8、一种以酿酒酵母孢子作为新型吸附剂的制备方法及应用，201310420997.1，高晓冬，中西秀树，张海妮，李子杰，申请
三、承担教学科研项目情况
1、国家自然科学基金面上项目2项；
2、江南大学自主科研计划资助；
3、江苏省双创人才项目；
4、江苏省创新团队项目；
四、获奖情况（含指导学生获奖）
以上资料更新时间截止：2022年8月
